Thawatchai Chaijarasphong's Homepage

Dr. Thawatchai Chaijarasphong

Group : Industrial Biotechnology and Bioprocess Engineering

Contact Details :

Room BT110
Department of Biotechnology, Faculty of Science, Mahidol University
Rama VI Road, Bangkok 10400, Thailand
Tel : 0-2201-5880 Fax : 0-2354-7160
E-mail : thawatchai.chi@mahidol.ac.th

Academic Position :

Lecturer, Department of Biotechnology, Faculty of Science, Mahidol University

Education :

	2007-2011 B.S. in Chemistry with Distinction, Stanford University, CA, USA.
	2011-2017 Ph.D. in Chemistry (Chemical Biology), University of California at Berkeley, CA, USA.

Research Experiences :

	2008 Stanford University, CA, USA Advisor: Professor Barry M. Trost • Optimization of ligands for a chemoselective catalyst involved in total synthesis of bryostatin 16
	2009-2010 Stanford University, CA, USA Advisor: Professor Bianxiao Cui • Investigation of axonal transport of Rab7 GTPase in rat cortical neurons
	2011-2017 University of California at Berkeley Berkeley, CA, USA Graduate Student Researcher Advisor: Professor David F. Savage • Investigations of the mechanism whereby a single csoS2 gene produces two CsoS2 isoforms and the roles of CsoS2 in self-assembly of the carboxysome, a proteinaceous organelle • Directed evolution of aldehyde-deformylating oxygenase to produce branched and short-chain alkanes

Teaching Experiences :

	2010 Stanford University, CA Chemistry tutor for students with learning disabilities
	2011-2012 University of California at Berkeley, CA Graduate Student Instructor, Organic Chemistry Laboratory for Chemistry Majors (CHEM 112A)
	2013-2015 University of California at Berkeley, CA Graduate Student Instructor, Chemical Structure and Reactivity/Organic Chemistry Laboratory (CHEM 3A/3AL)
	2017-present Lecturer at Department of Biotechnology, Faculty of Science, Mahidol University, Thailand

Teaching Experiences :

	07/2005 Silver medal, the 37th International Chemistry Olympiad
	07/2006 Silver medal, the 38th International Chemistry Olympiad
	08/2006 Royal Thai Scholarship
	03/2010 Selected by Bings Overseas Studies Program to study abroad at Doshisha University, Kyoto, Japan
	06/2011 Phi Beta Kappa
	06/2011 The Merck Index Award for an outstanding chemistry major at Stanford University(based on scholastic achievement)
	03/2016 RNA Society travel grant to present at the 2016 RNA Society Conference

Research Interest :

- Development of CRISPR-Cas based shrimp disease detection methods
- Application of techniques in molecular biology to engineer shrimp's pathogen resistance and develop disease detection platforms
- Protein engineering for improvement of physical properties of shrimp therapeutic proteins

Publications :
(Recent Articles from Scopus at MUSC Expertise: please click)

Luke Oltrogge, Thawatchai Chaijarasphong, Allen Chen, Eric Bolin, Susan Marqusee, David Savage. α-carboxysome formation is mediated by the multivalent and disordered protein CsoS2. Nature Structural & Molecular Biology. Submitted

Thawatchai Chaijarasphong, Thanyawit Thammachai, Ornchuma Itsathitphaisarn, Kallaya Sritunyalucksana & Rungkarn Suebsing. (2019). Potential application of CRISPR-Cas12a fluorescence assay coupled with rapid nucleic acid amplification for detection of white spot syndrome virus in shrimp. Aquaculture. doi:10.1016/j.aquaculture.2019.734340

Werawich Pattarayingsakul, Arnon Pudgerd, Natthinee Munkongwongsiri, Rapeepun Vanichviriyakit, Thawatchai Chaijarasphong, Siripong Thitamadee, & Thanapong Kruangkum. (2019). The gastric sieve of penaeid shrimp species is a sub-micrometer nutrient filter. The Journal of Experimental Biology, 222(10). doi:10.1242/jeb.199638

Thawatchai Chaijarasphong & David Savage (2018). Sequestered: Design and Construction of Synthetic Organelles. Synthetic Biology: Parts, Devices and Applications, 279-306. doi:10.1002/9783527688104.ch14

Robert Nichols, Caleb Cassidy-Amstutz, Thawatchai Chaijarasphong, David Savage (2017). Encapsulins: Molecular biology of the shell. Critical Reviews in Biochemistry and Molecular Biology, 52(5), 583-594. doi:10.1080/10409238.2017.1337709

Thawatchai Chaijarasphong, Robert Nichols, Katie Kortright, Charlotte Nixon,PohTeng, Luke Oltrogge, David Savage. “Programmed Ribosomal Frameshifting Mediates Expression of the α-Carboxysome”, Journal of Molecular Biology. 2016, 428(1), 153–164.

Oral presentations :

“Order and Chaos: How CsoS2, an Intrinsically Disordered Protein, Organizes the Assembly of a Bacterial Organelle,” presented at Department of Microbiology, Chulalongkorn University, Bangkok, Thailand (June 2016)

“Order and Chaos: How CsoS2, an Intrinsically Disordered Protein, Organizes the Assembly of a Bacterial Organelle,” presented at the 2016 University of California Symposium of Chemical Sciences (UCSCS), San Bernardino, CA, USA (March 23, 2016)

“Exploring Substrate Specificity and Redox Partners of an Aldehyde-Deformylating Oxygenase from Nostocpunctiforme for Alkane Production,” presented at Graduate Research Seminar, UC Berkeley, Berkeley, CA, USA (April 25, 2013)

Poster presentations :

Thawatchai Chaijarasphong, Robert J. Nichols, Luke M. Oltrogge, and David F. Savage. “Programmed ribosomal frameshifting mediates the assembly of a CO2-fixing bacterial organelle,” scheduled to present at the Joint Meeting of The RNA Society and RNA Society of Japan, Kyoto, Japan (June 28 – July 2, 2016)

Thawatchai Chaijarasphong, Robert J. Nichols, Luke M. Oltrogge, and David F. Savage. “CsoS2 undergoes -1 programmed ribosomal frameshifting to generate two isoforms and is involved in α-carboxysome assembly,” scheduled to present at Chemical Biology in the Bay Area (CBBA) Day, UC Berkeley, California, USA (April 30, 2016)

Thawatchai Chaijarasphong, Robert J. Nichols, Luke M. Oltrogge, and David F. Savage. “CsoS2 undergoes -1 programmed ribosomal frameshifting to generate two isoforms and is involved in α-carboxysome assembly,” presented at UC Berkeley Biochemistry, Biophysics, and Structural Biology (BBS) retreat, Asilomar, California, USA (January 14, 2016)

Thawatchai Chaijarasphong, Poh K. Teng, and David F. Savage. “CsoS2 is an intrinsically disordered protein involved in H. neapolitanuscarboxysome assembly” presented at 2014 Synberc annual conference, MIT, Cambridge, MA, USA (September 28, 2014) ^th congress on science and technology (STT37), October 10-12, 2011, Centara Grand & Bangkok Convention Centre at Central World, Bangkok, Thailand.

Poster presentations :

• Protein expression and purification: protein overexpression in E. coli, Nickel affinity purification, Strep-Tactin affinity purification, size-exclusion chromatography, ion-exchange chromatography

• Protein-protein interaction analysis: microscale thermophoresis (MST), bio-layer interferometry (BLI), bacterial two-hybrid assay, co-immunoprecipitation, native PAGE, fluorescent anisotropy, Förster resonance energy transfer (FRET), chemical crosslinking

• Protein detection and characterization: SDS-PAGE, Western blotting, MALDI-TOF, circular dichroism (CD), dynamic light scattering (DLS)

• Cloning and genetic manipulation: plasmid and genomic DNA isolation from various bacteria, DNA ligation via traditional ligation, Golden-Gate assembly, and Gibson assembly; error-prone PCR, site-directed mutagenesis, plasmid library construction, P1 phage transduction,gene replacement by homologous recombination, bacterial transformation via electroporation and heat-shocking

• Experience with microorganisms: Escherichia coli, Synechococcuselongatus PCC7942, Halothiobacillusneapolitanus

• Optical techniques: fluorescent microscopy, fluorescent recovery after photobleaching (FRAP)

• Other: purification of bacterial organelles via sucrose-gradient ultracentrifugation, carbon-dioxide fixation assay, organic synthesis, infrared spectroscopy, small-molecule NMR