Dr. Thapakorn Jaroentomeechai
 Group : Industrial Biotechnology and Bioprocess Engineering
 
Contact Details :
Room BT207
Department of Biotechnology, Faculty of Science, Mahidol University
Rama VI Road, Bangkok 10400, Thailand
Tel : 0-2201-5317 Fax : (66-2) 354-7160
E-mail : thapakorn.jar@mahidol.ac.th
Current Academic Appointment :
Lecturer, Department of Biotechnology, Faculty of Science, Mahidol University
Research Interest :
- cell-free platform, synthetic biology, glycoengineering, therapeutic glycoprotein production
- SynGlyco Research Group webpage
Education :
2021 Ph.D. in Chemical Engineering, Cornell University, Ithaca, NY
2014 M.Eng. in Chemical Engineering, Cornell University, Ithaca, NY
2012 B.S. in Chemistry (Summa Cum Laude), Mahidol University, Bangkok, Thailand
Research Experience :
Postdoctoral Researcher, Copenhagen Center for Glycomics, University of Copenhagen, Denmark (2021-2025)
Professor Henrik Clausen, University of Copenhagen, Denmark
• High throughput hybridoma screening for anti-carbohydrate antibody discovery using ELISA, flow cytometry screening of the reporter gene assays
• Application of cell-based array to probe antibody specificity and affinity
• Develop single cell antibody discovery for immunodominant epitope identification in cancer patients
• Develop mammalian cell-based production platform for single cell glycomics development
• Structural and functional interrogations of carbohydrate binding proteins
• Mass spectrometry analysis of glycan, glycopeptide, and glycoproteins
• Single molecule analysis of glycosylated biomolecules

Research Assistant, Chemical Engineering, Cornell University, Ithaca, NY (2014-2021)
Professor Matthew P. DeLisa, Cornell University, USA
• Developed a robust tool for producing glycoproteins in a cell-free system
• Adopted in vitro glycosylation as a tool to interrogate glycosylation-related enzymes
• Improved synthesis yields of glycoproteins in E. coli using a metabolic engineering approach
• Designed a cell-free glycosylation system for personalized glycovaccines and decentralized biomanufacturing
• Designed, developed, and tested cell-free glycosylation in a microfluidic device for bio-nanomanufacturing
• Applied protein engineering strategies to improve the solubility and activity of glycosyltransferases
• Applied glycoengineering strategy to increase pharmacological profile of therapeutic antibodies
• Mentored several junior researchers including high school, undergraduate, master’s, and PhD rotation students

Research CO-OP, Antibody Discovery Group, Biogen, Inc. (2020-2021)
Dr. Hua Wang, Research Scientist, Antibody Discovery, Biogen, USA
• Isolated and immunoprofiled of murine lymphocytes including memory and plasma B-cells using multicolor flow cytometry
• Isolated antigen-specific B-cells from immunized mice
• Prepared next-generation sequencing samples (NGS) and analyzed NGS data to validate antibody germlines present in humanized mice model
• Developed single B-cell cloning strategy for accelerated antibody discovery pipeline
• Adopted cytometry-based cell-surface antigen quantification technique to facilitate cell-based hybridoma screening
• Integrated single cell cloning with hybridoma screening scheme to improve antibody discovery pipeline
• Performed germline alignment and VH/VL pairing from the antibody sequence repertoires retrieved
• Maintained and created user protocol for flow cytometry instrument

Research Assistant, Chemistry and Chemical Biology, Gunma University, Japan (2012)
Professor Dr. Masafumi Unno, Gunma University, Japan
• Devised a synthesis strategy for preparing a gram-quantity of cage-siloxanes building blocks

Research Assistant, Chemistry, Mahidol University, Bangkok, Thailand (2011-2013)
Professor Vuthichai Ervithayasuporn, Mahidol University, Thailand
• First to report N--nucleophile-induced cage-rearrangement of the silsesquioxane core
• Investigated halogen exchange reaction as a facile route to prepare silsesquioxane molecules
• Synthesized highly-acidic silsesquioxane as a metal-free catalyst to prepare polycaprolactone
• Designed experiments for Inorganic Chemistry Laboratory Class • Mentored junior undergraduate researchers
Professional experience and memberships :
2025-present Editorial Board, Glycoconjugate Journal  
  2025-present Consultant, Fundação para a Ciência e a Tecnologias (project 2024.15575.PEX)    
  2025 Grant reviewer for the Croation National Science Foundation 
  2023-present Review Editor, Frontier in Chemistry (Glycoscience section)
  2023 Teaching Panel, European Innovative Action in Glycoscience, workshop 2, Erice, Italy
  2021-present Member, Society for Biological Engineering
  2020-present Member, Society for Glycobiology
  2018 Member, American Institute of Chemical Engineers
Grants and awards :
2024 NEYE Fonden Grant Award for research into cancer and prevention of the disease (DKK208,000)        
2023 Travel Award, International Symposium in Glycoconjugates, Taipei, Taiwan
  2021 European Molecular Biology Organization (EMBO) Postdoctoral Fellowships Award (~EUR175,000)
  2020 Austin Hooey Graduate Research Excellence Award, Cornell University
  2019 CBE Fleming Scholar Award (~USD45,000), Cornell University
  2013 Royal Thai Government Scholar Award
  2013 Outstanding undergraduate research award in Mahidol University, Thailand
  2013 Dean’s list, Department of Chemistry, Mahidol University, Thailand
  2012 Presentation award in the 7th Conference on Science and Technology for Youth, Thailand
  2008 Mahidol University Young Scientist Scholar (Sri Trang Thong), Thailand
  2004 National Junior Science Talent Project Scholar, Thailand
Professional training :
2025 Communication Research Workshop, EMBO Solutions GmbH 
2024 Introduction to University Pedagogy, Fall 2024 cohort, University of Copenhagen
  2022 EMBO Laboratory Leadership October 2022 cohort
  2021 Research integrity (Core & Supplement courses), Epigeum Online Course System
Peer-reviewed publications :
Total: 25 with 7 first/co-first authorships.
H-index = 18, Citation = 1178 (Google scholar – 2025.10.16), * indicates equal contributions
   
Highlighted Publications
• Jaroentomeechai , T, Kwon, YH, Liu, Y, Young, O, Bhawal, R, Wilson, JD, Li, M, Chapla, DG, Moremen, KW, Jewett, MC, Mizrachi, D, and DeLisa, MP (2022) A universal glycoenzyme biosynthesis pipeline that enables efficient cell-free remodeling of glycans. Nat Commun 13: 6325.
- First report protein engineering strategy for universal production of human glycosyltransferases in E. coli
- 50 Best articles, feature article in Editors’ Highlights webpage of recent research in “Biotechnology and methods” section of Nature Communication.

• Stark, JC*, Jaroentomeechai, T*, Moeller, TD, Dubner, RS, Hsu, KJ, Stevenson, T, DeLisa, MP, and Jewett, MC (2021) On-demand, cell-free biomanufacturing of conjugate vaccines at the point-of-care. Sci Adv 7: 1-15.
- First report the application of cell-free glycosylation system in biomanufacturing of conjugate vaccine
- Cover page for the journal in February 2021, vol 7, issue 6.
- Highlighted in the Cornell Chronicle February 3, 2021.
- Highlighted in Sciencemag February 3, 2021.
- Highlighted in Technology Networks February 4, 2021.

• Natarajan, A, Jaroentomeechai, T, Cabrera, M, Mohammed, J, Cox, EC, Young, O, Shajahan, A, Vilkhovoy, M, Vadhin, S, Varner, JD, Azadi, P and DeLisa, MP (2020) An orthogonal O-linked protein glycosylation pathway in bacteria yields tumor-associated glycotopes. Nat Chem Biol 16: 1062–1070.
- First report of bacterial cell-free system capable of performing protein O-glycosylation
- Highlighted in the Cornell Chronicle September 15, 2020.

• Jaroentomeechai, T*, Stark, JC*, Natarajan, A, Glasscock, CJ, Yates, LE, Hsu, KJ, Mrksich, M, Jewett, MC and DeLisa, MP (2018) Single-pot glycoprotein biosynthesis using a cell-free transcriptiontranslation system enriched with glycosylation machinery. Nat Commun 9: 2686.
- First report of bacterial cell-free system capable of performing protein N-glycosylation
- Highlighted in Chemical & Engineering News (C&EN) on July 29, 2018, volume 96, issue 31 (Link) and on November 12, 2018, volume 96, issue 45.
- Highlighted in the Cornell Chronicle July 12, 2018.
- Highlighted as one of the technology to accelerate the development of COVID-19 therapeutics by the Cornell Chronicle, April 6, 2020 (Link) and the Northwestern Now, April 2, 2020.

Full Publication List. (* equal contributions, † corresponding author)

25. Jaroentomeechai T, Veloz B, Soares C, Goerdeler F, Grosso A, Büll C, Miller R, Furukawa S, Ginés- Alcober I, Taleb V, Merino P, Ghirardello M, Compañón I, Coelho H, Dias J, Vincentelli R, Henrissat B, Joshi H, Clausen H, Corzana F, Marcelo F, Hurtado-Guerrero R, Narimatsu Y (2025). Microbial binding module employs sophisticated clustered saccharide patches to selectively adhere to mucins. Nat Commun 16, 9058.

24. Chen J, Goerdeler F, Jaroentomeechai T, Hernandez FXS, Wang X, Clausen H, Narimatsu Y, Satchell KJF† (2025). Biantennary N-glycans As Receptors for MARTX Toxins in Vibrio Pathogenesis. Sci Adv 11, eadt0063

23. Puagsopa P, Tongviseskul N, Jaroentomeechai T†, Meksiriporn B† (2025). Recent progress in developing extracellular vesicles as nanovehicles to deliver carbohydrate-based therapeutics and vaccines. Vaccines 13, 3:285 Page 4 of 9

22. Jaroentomeechai T, Karlsson R, Goerdeler F, Teoh FKY, Grønset MN, de Wit D, Chen YH, Furukawa S, Hurtado-Guerrero R, Vidal-Calvo EE, Salanti A, Boltje TJ, van den Bos LJ, Wunder C, Johannes L, Schjoldager K, Joshi H, Miller R, Clausen H, Vakhrushev SY, Narimatsu Y† (2024). Mammalian Cell- Based Production of Glycans, Glycopeptides and Glycomodules. Nat Commun 15, 9668

21. Tóth ZS, Leveles l, Nyíri K, Nagy GN, Harmat V, Jaroentomeechai T, Ozohanics O, Miller RL, Álvarez MB, Vértessy BG, Benedek A† (2024). The homodimerization domain of the Stl repressor is crucial for efficient inhibition of mycobacterial dUTPase. Sci Rep 14, 27171

20. Luisa N. Pimentel-Vera LN, Rodríguez-López A, Espejo-Mojica AJ, Ramírez AM, Cardona C, Reyes LH, Tomatsu S, Jaroentomeechai T, DeLisa MP, Sánchez OF, Alméciga-Díaz CJ† (2024). Novel human recombinant N-acetylgalactosamine-6-sulfate sulfatase produced in a glyco-engineered Escherichia coli strain. Heliyon 10,12:32555

19. Anggara K, Laura Sršan L, Jaroentomeechai T, Wu X, Rauschenbach S, Narimatsu Y, Clausen H, Ziegler T†, Miller RL†, Kern K† (2023). Direct observation of glycans bonded to proteins and lipids at single molecule level. Science 382;6667:219

18. Stark JC†, Jaroentomeechai T, Warfel KF, Hershewe JM, DeLisa MP†, Jewett MC† (2023). Rapid Biosynthesis of glycoprotein therapeutics and vaccines from freeze-dried bacterial cell lysates. Nat Protoc 18:2374

17. Robinson MP, Jung J, Lopez-Barbosa N, Chang M, Li M, Jaroentomeechai T, Cox E, Zheng X, Berkmen M, DeLisa MP† (2023). Isolation of full-length IgG antibodies from combinatorial libraries expressed in the cytoplasm of Escherichia coli. Nat Commun 14:3514

16. Weyant KB, Liao J, Jesus MR-D, Jaroentomeechai T, Moeller TD, Hoang-Phou S, Pal S, Gilmore SF, Singh R, Putnam D, Locher C, de la Maza LM, Coleman MA, DeLisa MP† (2023). A modular platform for on-demand vaccine self-assembly enabled by decoration of bacterial outer membrane vesicles with biotinylated antigens. Nat Commun 14: 464

15. Jaroentomeechai T, Kwon YH, Liu Y, Young O, Bhawal R, Wilson JD, Li M, Chapla DG, Moremen KW, Jewett MC, Mizrachi D, DeLisa MP† (2022). A universal glycoenzyme biosynthesis pipeline that enables efficient cell-free remodeling of glycans. Nat Commun 13: 6325

14. Li M, Zheng X, Shanker S, Jaroentomeechai T, Moeller TD, Hulbert S, Koçer I, Byrne J, Cox EC, Labonte JW, Gray JJ, DeLisa, MP† (2021). Shotgun scanning glycomutagenesis: a simple and efficient strategy for constructing and characterizing neoglycoproteins. Proc Natl Acad Sci USA 118:e2107440118

13. Stark JC*, Jaroentomeechai T*, Moeller TD, Dubner RS, Hsu KJ, Stevenson TC, DeLisa MP†, Jewett MC† (2021). On-demand, cell-free biomanufacturing of conjugate vaccines at the point-of-care. Sci Adv 7:1-15

12. Natarajan A, Jaroentomeechai T, Cabrera M, Mohammed J, Cox EC, Young O, Shajahan A, Vilkhovoy M, Vadhin S, Varner JD, Azadi P, DeLisa, MP† (2020). An orthogonal O-linked protein glycosylation pathway in bacteria yields tumor-associated glycotopes. Nat Chem Biol 16:1062-1070 Page 5 of 9

11. Jaroentomeechai T, Taw MN, Li M, Aquino A, Agashe N, Chung S, Jewett MC, DeLisa MP† (2020). Cell-free synthetic glycobiology: designing and engineering glycomolecules outside the cell. Front Chem 8: 645

10. Ervithayasuporn V†, Chanmungkalakul S, Churinthorn N, Jaroentomeechai T, Hanprasit S, Sodkhomkhum R, Kaewpijit, P, Kiatkamjornwong S (2019). Modifying interlayer space of montmorillonite with octakis (3-(1-methylimidazolium) propyl) octasilsesquioxane chloride. Appl Clay Sci 171:6-13

9. Natarajan A, Jaroentomeechai T, Li M, Glasscock CJ, DeLisa, MP† (2018). Metabolic engineering of glycoprotein synthesis in bacteria. Emerg Top Life Sci 2:419-432

8. Jaroentomeechai T*, Stark JC*, Natarajan A, Glasscock CJ, Yates LE, Hsu KJ, Mrksich M, Jewett MC†, DeLisa MP† (2018). Single-pot glycoprotein biosynthesis using a cell-free transcription-translation system enriched with glycosylation machinery. Nat Commun 9:2686

7. Glasscock CJ, Yates LE, Jaroentomeechai T, Wilson JD, Merritt JH, Lucks JB, DeLisa, MP† (2018). A flow cytometric approach to engineering Escherichia coli for improved eukaryotic protein glycosylation. Metab Eng 47:488-495

6. Schoborg JA, Hershewe JM, Stark JC, Kightlinger WK, Kath JE, Jaroentomeechai T, Natarajan A, DeLisa, MP, Jewet MC† (2018). A cell-free platform for rapid synthesis and testing of active oligosaccharyltransferases. Biotechnol Bioeng 115:739-750

5. Jaroentomeechai T, Zheng X, Hershewe J, Stark JC, Jewett MC, DeLisa, MP† (2017). A pipeline for studying and engineering single-subunit oligosaccharyltransferases. Methods Enzymol 597:55-81

4. Ollis AA, Chai Y, Natarajan A, Perregaux EC, Jaroentomeechai T, Guarino C, Smith J, Zhang S, DeLisa MP† (2015) Substitute sweeteners: diverse bacterial oligosaccharyltransferases with unique Nglycosylation site preferences. Sci Rep 5: 15237

3. Ervithayasuporn V†, Pornsamutsin N, Prangyoo P, Sammawutthichai K, Jaroentomeechai T, Phurat C, Teerawatananond T (2013). One-pot synthesis of Halogen exchanged silsesquioxanes: Octakis(3- bromopropyl) octasilsesquioxane and Octakis(3-iodopropyl) octasilsesquioxane. Dalton Trans 42:13747-13753

2. Jaroentomeechai T, Yingsukkamol P, Phurat C, Somsook E, Osotchan T, Ervithayasuporn V† (2012). Synthesis and Reactivity of Nitrogen Nucleophiles-Induced Cage-Rearrangement Silsesquioxanes. Inorg Chem 51:12266-12272

1. Ervithayasuporn V†, Tomeechai# T, Takeda N, Unno M, Chaiyanurakkul A, Hamkool R, Osotchan, T (2011) .Synthesis and Characterization of Octakis(3-propyl ethanethioate) octasilsesquioxane. Organometallics 30: 4475-4478 (# Tomeechai is a formal last name)1. Pongtharangkul, T., Chuekitkumchorn, P., Suwanampa, N., Payongsri, P., Honda, K., Panbangred, W., 2015. Kinetic properties and stability of glucose dehydrogenase from Bacillus amyloliquefaciens SB5 and its potential for cofactor regeneration. AMB Express 5, 68.

Patents and applications

1. Jewett MC, Stark JC, DeLisa MP, Jaroentomeechai T. Method for rapid in vitro synthesis of bioconjugate vaccines via recombinant production of N-glycosylated proteins in prokaryotic cell lysates. United States Patent Application 20210139940, A1 - 05/13/2021.

2. Stark JC, Jaroentomeechai T, DeLisa MP, Jewett MC. Cell-free glycoprotein synthesis (CFGpS) in prokaryotic cell lysates enriched with glycosylation machinery. WO 207117539 A1.

3. Jewett MC, Stark JC, DeLisa MP, Jaroentomeechai T. Method for rapid in vitro synthesis of bioconjugate vaccines with FDA-approved protein carriers in detoxified prokaryotic cell lysates. US 20180016612 A1.

4. DeLisa MP, Jaroentomeechai T, Mizrachi D. Compositions and methods for solubilizing glycosyltransferases. US 147402.009111.

Peer review activity

1. During doctoral study (with Dr. Matthew DeLisa) – Biotechnology and Bioengineering; Nature Chemical Biology; ACS Chemical Biology; Journal of American Chemical Society
2. Independent review activity - Journal of Undergraduate Chemical Engineering Research; STAR Protocol; Frontier in Molecular Biosciences (Glycoscience section); Frontier in Immunology; Advanced Science; Frontier in Pharmacology; Frontiers in Plant Science;
3. Review Editor: Frontier in Chemistry – Glycoscience section (2023-present)
4. Editorial Board, Glycoconjugate Journal (2025-present)


Last updated : 9/12/2025

customizable counter

View My Stats
Copyright ©2025 Department of Biotechnology, Mahidol University.  All Rights Reserved
Web master : pravit.won@mahidol.ac.th