Dr. Thunyarat Pongtharangkul

Group : Industrial Biotechnology and Bioprocess Engineering

Contact Details :

Office: SC4/313 (Science Bldg. 3, Salaya Campus) Laboratory: R3/6 (Science Bldg. 3, Salaya Campus) Department of Biotechnology, Faculty of Science, Mahidol University, Bangkok, Thailand 10400 Mobile: (6686) 379-7037, Fax: (662) 354-7160 Email: thunyarat.pon@mahidol.ac.th

Position :

Assistant Professor

Professional Experiences :

Lecturer, Mahidol University, Bangkok, Thailand (2006-present) Teaching Assistance, The Pennsylvania State University, PA, USA (2002-2006) Graduate Research Assistance, Chulalongkorn University, Bangkok, Thailand (2000-2001)

 

Education :

Doctor of Philosophy (Agricultural and Biotechnological Engineering), The Pennsylvania State University, PA, USA Master of Science (Biotechnology), Chulalongkorn University, Bangkok, Thailand Bachelor of Science (Biotechnology), Chulalongkorn University, Bangkok, Thailand

Research Interest :

• Development of whole-cell biocatalysts for production of bio-based chemicals (e.g. epoxides, vanillin) • Production of value-added products via fermentation (e.g. bacteriocin, CRM197) • Aqueous/Organic (Biphasic) bioconversion • Bioremediation of toxic chemicals (e.g. BTEX, PAHs)

Publications :
(Recent Articles from Scopus at MUSC Expertise: please click)

Journal Articles

1.       Pongtharangkul T, Demirci A. Evaluation of agar well diffusion method for nisin quantification. ApplMicrobiolBiot. 2004 Aug;65(3):268-72.

2.       Pongtharangkul T, Demirci A. Evaluation of culture medium for nisin production in repeated-batch biofilm reactor. BiotechnolProgr. 2006a Jan-Feb;22(1):217-24.

3.       Pongtharangkul T, Demirci A. Effects of pH profiles on nisin production in biofilm reactor. ApplMicrobiolBiot. 2006b Aug;71(6):804-11.

4.       Pongtharangkul T, Demirci A. Effects of fed-batch fermentation and pH profiles on nisin production in suspended-cell and biofilm reactors. ApplMicrobiolBiot. 2006c Nov;73(1):73-9.

5.       Pongtharangku T, Demirci A. Online recovery of nisin during fermentation and its effect on nisin production in biofilm reactor. ApplMicrobiolBiot. 2007 Mar;74(3):555-62.

6.       Pongtharangkul T, Demirci A, Puri VM. Modeling of growth and nisin production by Lactococcuslactisduring batch fermentation. Biological Engineering 2008; 1(3): 265-75.

7.       Kongpol A, Pongtharangkul T, Kato J, Honda K, Ohtake H, Vangnai AS. Characterization of an organic-solvent tolerant Brevibacillusagri strain 13 able to stabilize solvent/water emulsion. FEMS Microbiol Lett. 2009 Aug;297:225-33.

8.       Rachadech W, Navacharoen A, Ruangsit W, Pongtharangkul T, Vangnai AS. An organic solvent-, detergent-, and thermo-stable alkaline protease from the mesophilic, organic solvent-tolerant Bacillus licheniformis 3C5. Mikrobiologiia. 2010 Sep-Oct;79(5):630-8.

9.       Wangrangsimagul N, Klinsakul K, Vangnai AS, Wongkongkatep J, Inprakhon P, Honda K, Ohtake H, Kato J, Pongtharangkul T. Bioproduction of vanillin using an organic solvent-tolerant Brevibacillusagri 13. ApplMicrobiolBiot. 2012 Jan;93(2):555-63.

10.    Wongkongkatep P, Manopwisedjaroen K, Tiposoth P, Archakunakorn S, Pongtharangkul T, Suphantharika M, Honda K, Hamachi I, Wongkongkatep J. Bacteria interface pickering emulsions stabilized by self-assembled bacteria-chitosan network. Langmuir 2012; 28(13):5729–36.

11.    Siriphongphaew A, Pisnupong P, Wongkongkatep J, Inprakhon P, Vangnai AS, Honda K, Ohtake H, Kato J, Ogawa J, Shimizu S, Urlacher VB, Schmid RD, Pongtharangkul T. Development of a whole-cell biocatalyst co-expressing P450 monooxygenase and glucose dehydrogenase for synthesis of epoxyhexane. ApplMicrobiolBiot. 2012 Jul;95(2):357-67.

12.    Chaichanan J, Wiyakrutta S, Pongtharangkul T, Israngkul D, Meevootisom V. Optimization of zofimarin production by an endophytic fungus, Xylariasp. ACRA L38. Brazilian J Microbiol. 2014; 19;45(1):287-93.

13.    Inprakhon P, Panlawan P, Pongtharangkul T, Marie E, Wiemann LO, Durand A, Sieber V. Toward one-pot lipase-catalyzed synthesis of poly(e-caprolactone) particles in aqueous dispersion. Colloids and Surfaces B: Biointerfaces. 2014; 113:254-60.

14.    Kongpol A, Kato J, Tajima T, Pongtharangkul T, Vangnai AS. Enhanced 3-methylcatechol production by Pseudomonas putida TODE1 in a two-phase biotransformation system. J Gen ApplMicrobiol. 2014; 60(5):183-90.

15.    Archakunakorn S, Charoenrat N, Khamsakhon S, Pongtharangkul T, Wongkongkatep P, Suphantharika M, Wongkongkatep J. Emulsification efficiency of adsorbed chitosan for bacterial cells accumulation at the oil-water interface. Bioprocess Biosyst Eng. 2015; 38:701-9. (

16.    Tiposoth P, Khamsakhon S, Ketsub N, Pongtharangkul T, Takashima I, Ojida A, Hamachi I, Wongkongkatep J. Rapid and quantitative fluorescence detection of pathogenic spore-forming bacteria using a xanthene-Zn(II) complex chemosensor. Sensors and Actuators B. 2015; 209:606-12.

17.    Kataoka N, Vangnai AS, Pongtharangkul T, Tajima T, Yakushi T, Matsushita K, Kato J. Construction of CoA-dependent 1-butanol synthetic pathway functions under aerobic conditions in Escherichia coli. J Biotechnol. 2015; 204:25-32.

18.    Pongtharangkul T, Chuekitkumchorn P, Suwanampa N, PayongsriP, Honda K, Panbangred W. Kinetic properties and stability of glucose dehydrogenase from Bacillus amyloliquefaciens SB5 and its potential for cofactor regeneration. AMB Express 2015; 5;68.

19.    Suwanpatcharakul M, Pakdeecharoema C, Visuttitewina S, Pesirikan N, Chauvatcharin S, Pongtharangkul T. Process Optimization for an industrial-scale production of Diphtheria toxin by Corynebacterium diphtheriae PW8. Biologicals 2016; 44(6):534-9.

20.    Wongthongdee N, Durand A, Pongtharangkul T, Sunintaboon P, Inprakhoe P. Green formulation strategy for preparing oil-in-water emulsions via lipase-catalyzed transesterification. ACS Sustainable Chemistry & Engineering 2017; 5(2):1948-56.

21.    Hanpanich O, Wongkongkatep P, Pongtharangkul T, Wongkongkatep J. Turning hydrophilic bacteria into biorenewable hydrophobic material with potential antimicrobial activity via interaction with chitosan. Bioresource Technology 2017; 230:97-102.

22.    Kataoka N, Vangnai AS, Pongtharangkul T, Yakushi T, Matsushita K. Butyrate production under aerobic growth conditions by engineered Escherichia coli. Journal of Bioscience and Bioengineering 2017; 123(5):562-8.

23.    Wongthongdee N, Durand A, Pongtharangkul T, Sunintaboond P, Inprakhon P. Lipase-catalyzed synthesis of sorbitol octanoate in aqueous biphasic medium and its use in a green formulation process of oil-in-water food nanoemulsions. Journal of Chemical Technology and Biotechnology 2017; 92(10):2650-60. DOI: 10.1002/jctb.5285.

24.    Wongbunmak A, Khiawjan S, Suphantharika M, Pongtharangkul T. BTEX- and naphthalene-degrading bacterium Microbacterium esteraromaticum strain SBS1-7 isolated from estuarine sediment. Journal of Hazardous Materials 2017;339:82-90.

25.    Kataoka N, Vangnai AS, Pongtharangkul T, Yakushi T, Matsushita K. Production of 1,3-diols in Escherichia coli. Bioresource Technology 2017; 245(Pt B):1538-41. DOI: 10.1016/j.biortech.2017.05.082.

26.    Inprakhon P, Wongthongdee N, Amornsakchai T, Pongtharankul T, Sunintaboon P, Wiemann LO, Durand A, Sieber V. Lipase-catalyzed synthesis of sucrose monoester: Increased productivity by combining enzyme pretreatment and non-aqueous biphasic medium. J Biotechnol 2017; 259:182-90. DOI: 10.1016/j.jbiotec.2017.07.021.

Book Chapter :

1.       Demirci A, Pongtharangkul T, Pometto III AL. Applications of biofilm reactors for production of value-added products by microbial fermentation. In: Blaschek HP, Wang H, Agle M, editors. Biofilms in the Food Environment. Ames, Iowa. Blackwell Publishing; 2007.

2.       Pongtharangkul T, Vangnai AS. Whole-cell biocatalysts from organic solvent-tolerant bacteria. หนังสือวิทยาศาสตร์เพื่อการพัฒนารวมบทความวิชาการฉลองครบรอบ50ปีแห่งการสถาปณาคณะวิทยาศาสตร์มหาวิทยาลัยมหิดล หน้า287-293; 2009. 

3.       Ercan D, Pongtharangkul T, Demirci A, Pometto III AL. Applications of biofilm reactors for production of value-added products by microbial fermentation. In: Pometto III AL, Demirci A, editors. Biofilms in the Food Environment. Ames, Iowa. Blackwell Publishing; 2015.

Paper in Proceedings :

1.       Chanarpakorn N, Pongtharangkul T. 2013. Partial characterization of glucose dehydrogenase
from Bacillus amyloliquefaciens strain SB5. Proceedings of the 27^th National Graduate Research Conference: A Path Towards National Development in the Context of ASEAN Community. February 28 – March 1. Naresuan University, Phitsanulok, Thailand.

2.       Khiawjan S*, Suphantharika M, Pongtharangkul T. 2014. Isolation of BTEX degrading bacteria from petroleum contaminated seabed sludges. Proceedings of the 2^nd ASEAN Plus Three Graduate Research Congress (2^nd AGRC), February 5-7. Bangkok, Thailand.

3.       Wangrangsimagul N*, Pongtharangkul T. 2014. Storage of a whole-cell biocatalyst. Proceedings of the 2^nd ASEAN Plus Three Graduate Research Congress (2^nd AGRC), February 5-7. Bangkok, Thailand.

4.       Charoenrat N*, Pongtharangkul T, JirarutWongkongkatep. 2014. Determination of bacterial cell viability in a 3D-scaffold. Proceedings of the 5^th RMUTP International Conference on Science Technology and Innovation for Sustainable Development, July 17-18. Bangkok, Thailand.

5.       Charoenrat N*, Pongtharangkul T, JirarutWongkongkatep. 2014. Assessment of bacterial cell viability in bacteria interface emulsion assisted by chitosan. Proceedings of the TSB International Forum 2014 “Green Bioprocess Engineering”, September 16-19. Bangkok, Thailand.

6.       Leiim J, Suphantharika M, Pongtharangkul T. 2015. Isolation and characterization of xylenes degrading-bacteria, Pseudomonas citronellolis PC1. Proceedings of The 27^th Annual Meeting of the Thai Society for Biotechnology and International Conference, November 18-19. Bangkok, Thailand.

7.       Sungwallek S, Payongsri P, Chaiyen P, Pongtharangkul T. 2016. Development of a whole-cell biocatalyst for caffeic acid production. Proceedings of The 28th Annual Meeting of the Thai Society of Biotechnology and International Conference, November 28-30. Chiang Mai, Thailand.

8.       Ekprasert, J., Kulthanawat, Y. and Pongtharangkul, T. 2016. Isolation and identification of styrene-degrading bacteria from polluted soils. Proceedings of The 28th Annual Meeting of the Thai Society of Biotechnology and International Conference, November 28-30. Chiang Mai, Thailand.

Oral Presentations :

1.       Pongtharangkul T, Demirci A.* 2004. Applications of biofilm reactors for production of value added products by microbial fermentation. The Institute of Biological Engineering (IBE) Annual Meeting. January 10. Fayetteville, AR.

2.       Pongtharangkul T*, Demirci A. 2005. Optimization of medium and pH control profile in biofilm fermentation for nisin production. ASAE Annual International Meeting. July 17-20. Tampa, FL.

3.       Pongtharangkul T*, Demirci A. 2005. Comparison of repeated batch and fed-batch fermentaion for nisin production in biofilm reactors with various pH control profiles. Northeast Agricultural and Biological Engineering Conference (NABEC). August 7-10. University of Delaware, Lewes, DL. (1^st Place in Graduate Student Competition)

4.       Pongtharangkul T*, Vangnai AS, Wongkongkatep J, Inprakhon P, Honda K*, Ohtake H. 2010. Bioconversion of water-immiscible chemicals. JSPS Asian Core Program (2009-2013): Next-generation bioproduction platform leveraging subtropical microbial bioresources. October 14-15. Institute of Microbiology and Biotechnology (IMBT), Hanoi, Vietnam.

5.       Pongtharangkul T*, Inprakhon P, Wongkongkatep J, Vangnai AS, Honda K, Ohtake H. 2012. Development of a whole-cell biocatalyst co-expressing the P450 monooxygenase and glucose dehydrogenase for synthesis of epoxyhexane. Annual Meeting of the Thai Society of Biotechnology (TSB2011). February 1-2. Imperial Queen’s Park Hotel, Bangkok, Thailand.

6.       Pongtharangkul T*. 2014. Development of whole-cell catalyst for synthesis of vanillin. Annual Symposium of TRF Team Building Grant (Senior Scholar) “Enzyme Catalysis”, October 10. Mahidol University, Bangkok, Thailand.

Professional Society Memberships :

• 2002-2003, Institute of Food Technologists (IFT) • 2002-2006, American Society of Agricultural and Biological Engineering (ASABE) • 2004-2010, American Society of Microbiology (ASM) • 2010-2011, The Society for Industrial Microbiology (SIM) • 2007-present, The Thai Society of Biotechnology (TSB)

Last updated : 9/8/2018